Qing-Hua-Yu-Re-Formula (QHYRF), a new herbal preparation, has been extensively used for treating diabetic cardiomyopathy.\nHowever, the chemical constituents of QHYRF remain uninvestigated. In the present study, rapid ultrahigh-performance liquid\nchromatography coupled with quadrupole-time-of-flight mass spectrometry (UHPLC-Q-TOF/MS) was used to qualitatively\nanalyze the components of QHYRF. Qualitative detection was performed on a Kromasil C18 column through the gradient elution\nmode, using acetonitrile-water containing 0.1% formic acid. Twenty-seven compounds were identified or tentatively characterized,\nincluding 12 phenolic acids, nine monoterpene glycosides, two flavonoids, three iridoids, and one unknown compound.\nAmong these, six compounds were confirmed by comparing with standards. A high-performance liquid chromatography (HPLC)\nmethod was developed to simultaneously determine the following six active components in QHYRF: danshensu, paeoniflorin,\nacteoside, lithospermic acid, salvianolic acid B, and salvianolic acid C. These HPLC chromatograms were monitored at 254, 280,\nand 320 nm. The method was well validated with respect to specificity, linearity, limit of detection, limit of quantification,\nprecision, stability, and recovery. The HPLC-UV method was successfully applied to 10 batches of QHYRF.
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